ORGLIST: Labeled nucleotides sticking to the surface of DNA-chips

Date view	Thread view	Subject view	Author view	Attachment view

From: Ralf Eisenhuth (ralf1977$##$googlemail.com)
Date: Mon May 26 2008 - 09:24:14 EDT

Next message: Rossana Ferrara: "ORGLIST: Dibenzylamine"
Previous message: Jarl Wikberg: "ORGLIST: Molecular exclusion chromatography of low mw compounds"

Hello everybody,

we have big issues with labelled monomers sticking on the surfaces of
different materials (like glas, gold or agarose...) during chemical primer
extention reactions. The monomers are either labled with Cy- or Bodipy-dyes
and have a 5'-phosphate group. Does anyone know of a (working) washing
protocoll to remove molecules like these without disturbing DNA duplex
stability ?

Best Regards

Ralf Eisenhuth

_______________________________________________
ORGLIST - Organic Chemistry Mailing List
Website / Archive / FAQ: http://www.orglist.net
To post a message (TO EVERYBODY) send to everybody$##$orglist.net
To unsubscribe, send to everybody-request$##$orglist.net the message: unsubscribe your_orglist_password your_address

Next message: Rossana Ferrara: "ORGLIST: Dibenzylamine"
Previous message: Jarl Wikberg: "ORGLIST: Molecular exclusion chromatography of low mw compounds"

Date view	Thread view	Subject view	Author view	Attachment view

This archive was generated by hypermail 2.1.4 : Wed Feb 11 2009 - 12:37:18 EST