Re: ORGLIST: Neutralizing TLC plates?

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From: Ohad Ilovich (ilovich$##$pob.huji.ac.il)
Date: Wed Jun 06 2007 - 01:20:07 EDT


Great idea Jason
I'll have to remember that trick for future use....
btw i've seen people running the whole TLC with 1% TEA and no just washing the silica beforehand with it to keep their materials stable. it hampers the TLC visualization in some systems but it does work with acid sesitive materials
Ohad
  ----- Original Message -----
  From: JASON SMITH
  To: everybody$##$orglist.net
  Sent: Tuesday, June 05, 2007 8:54 AM
  Subject: Re: ORGLIST: Neutralizing TLC plates?

  Stability on silica can be checked by running a 2D TLC:

  Using a square silica plate, spot the sample in one corner.

   Run the plate in one direction (all components of the sample will appear in a vertical line of spots).

   Turn the plate 90 degrees (your "line of spots" should be at the bottom) and run the plate again.

   If the sample is stable to silica, all spots will appear on the diagonal.

  -Jason

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  Ken Knott wrote:
    I have a compound that I think may be degrading on TLC plates due to the mild acidity.

    I would appreciate advice on how to neutralize TLC plates prior to running them. I understand that this can be done with a 1% solution of TEA in your eluent prior to running the compound? Can anyone offer any suggestions or advice on doing this?

    Also, how might I make myself more certain that I am in fact witnessing degradation of my compound as a result of running it on the TLC?

    Thanks in advance!

    Ken

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To unsubscribe, send to everybody-request$##$orglist.net the message: unsubscribe your_orglist_password your_addressYou may try passivating the TLC plate with hexamethyl disilazane (HMDS) via a vapor treatment. This procedure will methylate the SiO2. Of course, you will also have to functionalize your SiO2 if you run a column.

  Brandon

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