ORGLIST: TMS of wax esters

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From: Marilyn Vogel (mvogel$##$mail.arc.nasa.gov)
Date: Thu Jul 27 2006 - 15:17:19 EDT

I have another question about silylation of a wax ester fraction
taken bacterial extracts (e.g. mostly membrane lipids). I have used a
silylation method (pyridine + BSTFA, 1:1 vol/vol, 30 min @80C in a
teflon sealed vial), on a wax ester fraction collected from a TLC
plate and found TMS derivatives of carboxylic acids, methyl branched
carboxylic acids as well as primary and secondary alcohols. I have
check my TLC methods using standards so I am reasonably sure that I
am achieving good separation between my wax esters and alcohols, and
not combining material when I collect the fractions. Does anyone know
if BSTFA reacts with keto, ester or other functional groups on long
chain alkyl molecules?

Thanks,
Marilyn Vogel 

-- 
Marilyn Vogel, PhD
ORAU Postdoctoral Fellow
NASA Ames Research Center
Exobiology Branch, MS239-4
Moffett Field, CA 94035
Ph: 650.604.6941
Fax: 650.604.1088
mvogel$##$arc.mail.nasa.gov
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