From: Sengen Sun (sengensun$##$yahoo.com)
Date: Tue Apr 04 2006 - 17:23:48 EDT
--- fine chem <chemicals555$##$yahoo.com> wrote:
> Dear All,
>
> If a liquid sample consisting of a mixture of
> different soluble organic chemicals is analysed by
> HPLC how will one come to identify the individual
> consituents ?
> Can the probable constituents be subject to
> individual analyses (in known dosages) so as to
> confirm the identity and quantity ?
The only way as I know is to compare with standard
compounds you may have. If you don't have any
standard, you have to analyse each fraction you
collect by mass spectrometry (or LC-MS). If the scale
is large enough, you are doing a preparation
purification. You collect each fraction and analyse it
any way you want such as by proton NMR.
The more popular analysis is LC-MS instead of HPLC.
> Will this have to be done on the same HPLC
> instrument, using the same column, reagents,
> methods, etc ?
> Is there a data base library where one can compare
> the different peaks in the chromatogram so as to
> identify the probable substances ?
I don't think that there is any data base library
right now, although I heard such a thing when I was a
college student 25 years ago. I don't think that it is
useful any more as the large diversity of columns in
the market. Also, HPLC is wide used in analyse new
compounds that have never documented before.
Good luck,
Sengen
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