ORGLIST: HPLC chromatogram

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From: fine chem (chemicals555$##$yahoo.com)
Date: Tue Apr 04 2006 - 05:42:40 EDT


Dear All,
   
  If a liquid sample consisting of a mixture of different soluble organic chemicals is analysed by HPLC how will one come to identify the individual consituents ?
  Can the probable constituents be subject to individual analyses (in known dosages) so as to confirm the identity and quantity ?
  Will this have to be done on the same HPLC instrument, using the same column, reagents, methods, etc ?
  Is there a data base library where one can compare the different peaks in the chromatogram so as to identify the probable substances ?
  Will this differ from the column & methods used; is there a conversion factor ?
  Your assistance is much appreciated.
  jmarjorie
   
   

                
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