ORGLIST: Re: TLC for reactions in DMSO

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From: Sengen Sun (sengensun$##$yahoo.com)
Date: Fri Apr 15 2005 - 13:41:34 EDT


You may have to pump it under high vaccuum for 1-2 hrs
after you spot DMSO solution on the TLC plate. DMSO is
very hard to evaporate simply by blowing air on it.


Sengen Sun

--- srinivasa gopalan <arulmari$##$yahoo.com> wrote:

> Hi..
>
> TLC for reactions in DMSO
> yes, there indeed is a trick or rather two that i
> learnt from one of my mentors. try the follwing.
>
> 1. take an aliquot in a capillary or a small pastuer
> pipette - into a small test tube and blow the
> solvent
> dry using air - re-dissolve the residue in a low
> boiling solvent like acetonitrile or methanol and
> then
> spot.
>
> 2. if there are no suitable solvent to re-dissolve
> the above-residue, then spot directly onto the plate
> using thin capillary tubes then blow a stream of air
>
> on the TLC plate to dry the residual dmso before
> developing -
>
> these two tricks work like magic and also holds good
> for other high-boiling solvents like DMF and others.
>
> -Gopalan.
>
>
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> > 1. TLC of reactions run in DMSO (cardman)
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----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Thu, 14 Apr 2005 15:16:57 -0700 (PDT)
> > From: cardman <cardman400$##$yahoo.com>
> > Subject: ORGLIST: TLC of reactions run in DMSO
> > To: everybody$##$orglist.net
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<20050414221657.63341.qmail$##$web80903.mail.scd.yahoo.com>
> > Content-Type: text/plain; charset=us-ascii
> >
> > Are there any "tricks" for developing and
> > visualizing
> > spots when DMSO is used as a reaction solvent?
> DMSO
> > just seems to spread out on the plate rather than
> > evaporate, even after prolonged heating. As I
> result
> > I
> > often have huge spots that obscure a component or
> > two,
> > no matter what solvent system or adsorbent (normal
> > or
> > reverse phase) I use. The reaction components are
> > usually not UV-active.
> >
> >
> >
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