From: Javier Cuesta-Perez (javier$##$icr.ac.uk)
Date: Thu May 17 2001 - 10:04:06 EDT
Dear members,
I am currently making some phosphoramidites as building
blocks for oligonucleotide synthesis reacting the
appropriate alcohol with 2-cyanoethyl
N,N-diisopropylphosphoramidite and diisopropylethylamine,
which seems to be a standard procedure. As I can see in
some papers the normal work-up for this reactions includes
wash them with aqueous sodium bicarbonate and even
purification through SiO2 column chromatography. Is that
correct as a general rule? I mean, can I wash them with a
basic solution or pass them through a column without any
fear of hydrolysis or oxidation?
Many thanks,
Javier
_______________________________________
Dr Javier Cuesta-Perez
javier$##$icr.ac.uk
Biomolecular Structure Unit
The Institute of Cancer Research
15 Cotswold Rd
Sutton, Surrey
SM2 5NG, UK
Phone:(+44) (0)20 8643 8901 (ext. 4568)
Fax: (+44) (0)20 8643 1675
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