ORGLIST: FADH2 production and FAD assay

Date view Thread view Subject view Author view Attachment view

From: Joanne Jamie (joanne.jamie$##$mq.edu.au)
Date: Mon Dec 18 2000 - 23:09:02 EST

Dear all,

I am wanting to generate in situ FADH2 (the reduced form of flavin adenine
dinucleotide) from FAD. I need a system that is quite clean as I want to
test the ease of reduction of a series of organic compounds in the presence
of the FADH2 and do not wish for too many complicating species. I have seen
general references to use of NADH/NADPH and use of NaBH4. If anyone has
specific conditions that can be used that would be appreciated. Also
suggested assay methods for checking the activity of FAD would be useful.

Thank you

Joanne
 _________________________________________________________
 Dr Joanne Jamie Phone: (+61) (2) 9850 8283
 Deparment of Chemistry Fax: (+61) (2) 9850 8313
 Macquarie University E-mail: joanne.jamie$##$mq.edu.au
 Sydney, NSW, 2109
__________________

ORGLIST - Organic Chemistry Mailing List
Website / Archive / FAQ: http://www.orglist.net/
List coordinator: Joao Aires de Sousa (jas$##$mail.fct.unl.pt)

Date view Thread view Subject view Author view Attachment view

This archive was generated by hypermail 2.1.4 : Fri Sep 19 2003 - 12:15:35 EDT