From: Dr. Guillermo A. Morales (morales$##$combichemlab.com)
Date: Tue Jul 04 2000 - 10:28:57 EDT
Hi bin,
Well, I'm sure you already tried this, but one way to increase the loading
is trying double coupling.
If you tried the coupling using N-protected (Fmoc or tBoc) amino acid and
DCI in DCM and that didn't work well, another way to try is converting the
hydroxyl unit of the polymer support into a bromide using PPh3 and CBr4 in
DMF for 2-3 hours, then treat the brominated resin with N-protected amino
acid adding CsI or NaI in DMF or DMA.
I hope this helps.
Guillermo.
-----
Guillermo A. Morales
Morales Consulting
E-mail: morales$##$combichemlab.com
Website: http://www.combichemlab.com
Member of the Combi-Web Consortium: http://www.combi-web.com
-----Original Message-----
From: owner-orglist$##$dq.fct.unl.pt [mailto:owner-orglist$##$dq.fct.unl.pt]On
Behalf Of bin hu
Sent: Tuesday, July 04, 2000 7:13 AM
To: Multiple recipients of list orglist
Subject: None
hi!all you orglist members
Who is working in solid phase synthesis of peptide?Would you please
give me some advice in this field?
I am using Benzylamine Resin as solid support,HMBA(hydroxymethylated
benzoic acid) as Linker.I have met an problem in improving loading of the
first amino acid.
Thanking and waiting for your reply!
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