From: Rudi Riyanto (rudi76_1999$##$yahoo.com)
Date: Wed Feb 23 2000 - 01:56:45 EST
1. We had isolated chlorophylls from spinach leaves,
using this following method : after we got spinach
leaves extract / aceton, we added it with the
appropriate amount of CaCO3. The mixture was stirred
well and filtered using buchner flask. When the
filtrate was measured by spectrophotometri it showed
three peaks at 662, 430, 410 nm respectively as shown
in the literature. Is the isolated chloropyll pure, if
not do we need to do other tests such as TLC, paper
chromathography or other methods ?
2. By double beam spectrophotometer (UV-Vis Shimadzu
160A), how do we know the difference between
chlorophyll, pheophytin, Fe-pheophytin (heme),
Co-pheophytin ?
3. What's the colour of pheophytin, Fe-pheophytin,
Co-pheophytin ?
4. How do we get pheophytin, Fe-pheophitin,
Co-pheophytin from chlorophyll ? Can you explain it
more details ?
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