Re: ORGLIST: reverse phase LC

From: Christopher Paul Borella (cborella$##$ic.sunysb.edu)
Date: Fri Oct 22 1999 - 12:48:13 EDT

• Next message: Shu-Kun Lin: "ORGLIST: One-Wedge Convention for Stereochemical Representation, Proposal"
• Previous message: Vijay Sane: "ORGLIST: problem of disposal of side-products"
• In reply to: Bozell, Joe: "ORGLIST: reverse phase LC"
• Next in thread: Jonas Nilsson: "Re: ORGLIST: reverse phase LC"

We use a lyophalizer (freeze dryer) to remove the aqueous solvents from
our HPLC purifications. In general we remove the orgnic layer as best as
possible and then remove the aqueous with the freeze dryer, it can remove
about 2 liters of water overnight without much problem, newer systems
might be able to do this a bit faster. It eliminates extraction, and does
actually remove some buffers like NH4OAc, but you would have to check
about your specific buffers.

Hope this helps,

-Chris

__________________

ORGLIST - Organic Chemistry Mailing List
Website and Archive: http://www.orglist.net/
List coordinator: Joao Aires de Sousa (jas$##$mail.fct.unl.pt)

• Next message: Shu-Kun Lin: "ORGLIST: One-Wedge Convention for Stereochemical Representation, Proposal"
• Previous message: Vijay Sane: "ORGLIST: problem of disposal of side-products"
• In reply to: Bozell, Joe: "ORGLIST: reverse phase LC"
• Next in thread: Jonas Nilsson: "Re: ORGLIST: reverse phase LC"

This archive was generated by hypermail 2.1.4 : Fri Sep 19 2003 - 12:15:04 EDT