From: George D. McCallion (chem$##$bellatlantic.net)
Date: Wed Jan 20 1999 - 07:50:55 EST
My current work involves inositol, inositol phosphates, and
phospholipids (IP's). To separate the desired IP's, I use TLC. To
rermove the IP's from the silica, I use the same develping solvent;
nothing new here. My systems does consist of MeOH, CHCl3, and 4N
NH4OH. I use heat to concentrate the solutions during their elution
from the column.
My question is: is it possible for a base hyrdolysis to occur that would
'possibly' separate inositol from the lipid itself? It is true that
inositols are sensitive to alkali, but I wonder if the removal of
MeOH:CHCl3 via heating, thus leaving on 4N NH4OH, could induce cleavage.
Thank you in advance.
Sincerely,
--
George D. 'Merlin' McCallion, Research Chemist
Division of Metabolism
The Children's Hospital of Philadelphia
Post Office Box 143
Bala-Cynwyd, PA 19004-0143
Office: 1.215.590.6894
Fax: 1.215.590.3364
Email: chem$##$bellatlantic.net
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