ORGLIST: TLC question

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From: by way of Joao Aires de Sousa (chem$##$bellatlantic.net)
Date: Thu Nov 06 1997 - 19:02:39 EST



I will be involved in an upcoming research project that will initiate
with the following protocol:

                    Preparation of deuterated PtdIns and PtdIns-3,4,5-P3
using deuterated myo-inositol,
                    and exchange isolated enzymes through
chromatographic separation and purification.

I will be using (micro) TLC first, then (prep.) TLC which will allow us
to go onto HPLC (hopefully). My question to those who read this is: are
there any documented procedures, references, etc. on these experiments?
If so, please respond directly.

Thank you in advance.

Sincerely,
--
George D. McCallion, Research Chemist
Temple University
Post Office Box 143
Bala-Cynwyd, PA 19004-0143
United States

E-mail: chem$##$bellatlantic.net
Fax: 1.215.551.3990
Voice: 1.215.551.5336


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